The concentrations of sTREM-1, IL-1β, TNFα and IL-6 in cell-free culture supernatants were measured using commercially available specific ELISA kits (DuoSet, R&D Systems) according to the manufacturer's instructions. The detection limits were 31.3 pg/ml (sTREM-1 and IL-6), 15.6 pg/ml (TNFα) and 3.91 pg/ml (IL-1β).

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