C6, a rat glioma cell line, was procured from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in tissue culture flasks and grown in Ham's F12 medium (Sigma Aldrich) supplemented with 10% Fetal bovine serum (FBS; Gibco, New Zealand), and 1% antibiotic antimycotic solution (Sigma-Aldrich) in a humidified atmosphere of air and 5% CO2 at 37 °C.

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