The Cell Counting Kit-8 (CCK8) assay was employed to assess the number of viable cells and as a measure of the proliferation index. HK-2 cells were plated in 96-well plates at a density of 1×105 cells/well and then cultured in complete medium with or without LPS (100 ng/ml). The optical density at 450 nm (OD450) was determined at each time point.

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