Statistical analysis
This protocol is extracted from research article:
The expression and clinical significance of TPM4 in hepatocellular carcinoma
Int J Med Sci, Jan 1, 2021; DOI: 10.7150/ijms.49906

SPSS 20.0 software (SPSS, Chicago, IL, USA) was used to complete statistical estimations. Data was expressed as mean ± standard deviation with no less than three independent experiments included. Optimal cut-off point of TPM4 positive was obtained by ROC being introduced. Applied T-test was used to search for expression distinction between two groups. The chi-square served as a role to evaluate the connection between TPM4 expression and the clinicopathological parameters of hepatoma. If P values were less than 0.05, a distinction would be made as statistically significant.

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