22Rv1 cells were seeded in a 6-well plate at a density of 250×103 cells/well and incubated overnight at 37 °C. The cells were treated with HMC-FMX(DXT), HMC-FMX, or PBS. After 72 h, media was removed from the wells, and the cells were harvested by trypsinization. Cells were rinsed twice with PBS and resuspended in 400 μL of binding buffer (1X). The cell suspensions were treated with 5 μL of 7-AAD and PE annexin V, followed by incubation in the dark at RT for 15 min. The percent of early apoptosis and that of late apoptosis were measured by LSR Fortessa flow cytometry (BD Biosciences, San Jose, CA).

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