A total of 5×104 cells/mL were seeded per well of a 96-well plate and incubated overnight to allow for cell attachment and recovery. At the end of each incubation period, cells were treated with different concentrations of res-loaded nanoparticles for the concentrations and time points described in the figure legends. Cell viability was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-monotetrazolium bromide assay. At the end of each incubation period, 20 μL of MTT dye (1 mg/mL; Sigma St. Louis, MO) was added in each well and the plate was incubated at 37 °C for 4 hours. Subsequently, the plates were read on a microplate reader (Wallac, PerkinElmer, Massachusetts, USA) at 570 nm. Absorbance was proportional to the number of viable cells per well. Percentage of cell viability in each group was calculated after normalization to its own control. All data are presented as mean values±standard deviation and representative for at least two independent experiments performed in triplicates.

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