After culturing 22Rv1, PC3, DU145, LNCaP and RWPE-1 cells overnight at 37 °C, the cells were treated with HMC-FMX at 8.0 μg/mL [HMC] for 3 h. Medium was removed from the wells and rinsed twice with PBS. Cells were then fixed with 4% PFA at RT for 10 min and stained with DAPI (300 nM in PBS) for 15 min at RT. The cells were imaged using a fluorescence microscope (Keyence BZ-X710, Keyence, Osaka, Japan).

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