Total RNA from cultured cells was isolated using TRIzol Reagent (Invitrogen) following standard protocol. RNA quality was assessed using a spectrophotometer (NANOPhotometer® NP80, IMPLEN) and a 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit, Waldbronn, Germany); RNAs with a 260:280 ratio of ≥1.5 and an RNA integrity number of ≥8 were subjected to deep sequencing.

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