MCF-7, MDA-MB-231 and MCF-10A cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). MCF-7 and MDA-MB-231 breast cancer cell lines were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic/antimycotic. MCF-10A immortalized breast cell line was cultured in DMEM F12 supplemented with 20 ng/mL EGF, 100 ng/mL Cholera Toxin, 500 ng/mL Hydrocortizone, 10 μg/mL Insulin, 5% Horse Serum (HS) and 1% antibiotic/antimycotic. Sub culturing of the cells was performed using 0.25% trypsin. The RPMI, FBS, antibiotics and trypsin used in cell culture were purchased from Gibco, Invitrogen (Carlsbad, California, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.