MCF-7, MDA-MB-231 and MCF-10A cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). MCF-7 and MDA-MB-231 breast cancer cell lines were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic/antimycotic. MCF-10A immortalized breast cell line was cultured in DMEM F12 supplemented with 20 ng/mL EGF, 100 ng/mL Cholera Toxin, 500 ng/mL Hydrocortizone, 10 μg/mL Insulin, 5% Horse Serum (HS) and 1% antibiotic/antimycotic. Sub culturing of the cells was performed using 0.25% trypsin. The RPMI, FBS, antibiotics and trypsin used in cell culture were purchased from Gibco, Invitrogen (Carlsbad, California, USA).

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