In vivo and ex vivo fluorescence imaging has been carried out 24 hours post EV treatments using IVIS Lumina II Quantitative Fluorescent Imaging (PerkinElmer, Waltham, MA, USA) with suitable filters (Cy5.5) and following the manufacturer's instructions for fluorescence background subtraction. Mice were anaesthetized using Isofluorane (Isofluorane-Vet; Merial, Lyon, France) and kept under anesthesia during imaging sessions carried out with the Imaging System (5 min for dorsal view and 5 min for ventral view) (IVIS Lumina II Quantitative Fluorescent and Bioluminescent Imaging; PerkinElmer, Waltham, MA, USA). Photon emission in selected body areas was measured using the Living Image Software 3.2 (PerkinElmer). For the ex vivo imaging, mice were treated with luciferin 15 min prior euthanasia by cervical dislocation and ex vivo imaging of the selected organs were carried out immediately after death. The quantification was done with Living Image Software 3.2 (PerkinElmer).

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