RNAs from 5 combined liver samples from each ND-con and ND-inf groups (10 ± 1 S. japonicum cercariae, 9 weeks post infection) were isolated using RNAiso Plus kits (TaKaRa Biotechnology Co. Ltd.) following the manufacturer's protocol. After assessing the RNA integrity and concentration on a NanoDrop Ultramicro-Spectrophotometer (Thermo Fisher Scientific), the samples were labeled using the miRNA Complete Labeling and Hyb Kit (Agilent technologies, Santa Clara, CA, US) followed the manufacturer's instructions, labeling section. Each slide was hybridized with 100ng Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit (Agilent technologies) in hybridization Oven (Agilent technologies) at 55 ℃, 20 rpm for 20 hours according to the manufacturer's instructions, hybridization section. After hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Agilent technologies). Slides were scanned by Agilent Microarray Scanner (Agilent technologies) and Feature Extraction software 10.7 (Agilent technologies) with default settings. Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies). The results (Figure (Figure1A1A and Table S1) have been deposited in the Database of Genotypes and Phenotypes (dbGAP) of the National Center for Biotechnology Information (United States National Library of Medicine, Bethesda, MD, USA) under accession number GSE134866.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.