Twelve 6-week-old male C57BL/6 mice that had been maintained on a high-fat diet (45% kcal fat, 35% kcal carbohydrate, and 20% kcal protein; Research Diets Inc.) for 1 month were randomly divided into two groups: a high-fat diet-chronic infection group (HFD-inf) and the control group (HFD-con). The method of infection was the same as described above, and mice were sacrificed 9 weeks after the infection.

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