Forty-eight 6-week-old male C57BL/6 mice were separated randomly into two groups and fed a normal diet (ND) containing 10% kcal fat (Research Diets Inc., New Brunswick, NJ, USA). Snails were placed in deionized water and exposed to incandescent light for 3-4 h for cercarial release. For infection, the cercariae were counted and placed on glass cover slips by a 10 μl bacteriological loop 49. Each mouse in the experimental group (ND-inf) was infected with 10 ± 1 S. japonicum cercariae via shaved abdomen and the other unchallenged group was used as the normal control (ND-con). Eight randomly selected mice from each group were sacrificed at 3, 6, 9, and 12 weeks post-infection.

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