The signal-to-noise ratio of CUT&Tag-seq is generally much higher than the ChIP-seq. Therefore, in order to detect PEG10 protein-coupled DNA fragments, the CUT&Tag Kit (Vazyme) was used in accordance with the manufacturer's instructions. 1×104 cells were harvested with ConA beads and then cells were sequentially incubated with PEG10 antibody, a secondary antibody and pA-Tn5. PCR was used for library preparation after fragmenting and extracting DNA. The library was sequenced with HiSeq PE150 platform. Antibody messages are listed in the supplementary materials as Table S2.

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