A total of 2 × 105 MRC5 were suspended in 100 μL DMEM. Then the cell suspension was mixed with 100 μL of collagen mix containing 68.75 µL DMEM, 0.72 µL 1 N NaOH, and 31.25 µL Type 1 Rat Tail Collagen (Corning, NY, USA), and added to 1 well of 24-well plates and allowed to solidify for 45 min at 37 °C. After incubation with media containing tumor-derived exosomes, the gels were photographed at different time points. Image J software was employed to measure gel area and evaluates contraction.

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