The Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Fisher) was used (according to the manufacturer's instructions) to examine proteins coupled to IGF2BP1. Cells (1×107) were washed in PBS twice and then 500 μL IP lysis buffer (containing 1× Protease Inhibitor Cocktail) was added to lyse cells. One tenth of the supernatant was saved as the input. To the remaining supernatant, 5 μg flag antibody-magnetic bead complex was added and then samples were incubated at 4 °C, overnight. Beads were washed five times with buffer containing 10 μg/mL RNAse A before being eluted by 100 μL elution buffer. Protein samples (20 μL) were boiled in LDS buffer for western blotting, and 80 μL protein samples were analyzed by label-free LC/MS. All related antibody messages are provided in Table S2.

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