Cells were washed twice in pre-chilled PBS and lysed in a buffer (100 mmol/L NaCl, 50 mmol/L Tris [pH 8.0], 1% NP-40, 0.1% SDS, 0.5 mM EDTA and 1× proteinase inhibitor cocktail). Lysates were centrifuged at 12500 g. Supernatants were collected and proteins were denatured using an LDS buffer (Thermo Fisher). Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane. Membranes were blocked in 5% skimmed milk and sequentially incubated with primary and secondary antibodies. Antibody information is provided as supplementary materials, Table S2. The immunoblot was developed using Chemi-Doc (Bio-Rad) and ECL (Bio-Rad).

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