For overexpression of COL6A1 or STAT1 in OS cell lines, the full-length cDNA encoding COL6A1 was subcloned into the pENTER vector (Invitrogen). After virus packaged in HEK293T cells using Lipofectamine 3000, COL6A1, STAT1 or mock vectors were transfected into target cell lines. For knockdown of COL6A1 or STAT1, shRNA sequences targeting was cloned into a pLKO.1-TRC cloning vector. ShRNA-containing plasmids were packaged and transfected into target cell lines. Transfected cells used for overexpression or knockdown were selected with puromycin (5 µg/mL) for 2 weeks.

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