For overexpression of COL6A1 or STAT1 in OS cell lines, the full-length cDNA encoding COL6A1 was subcloned into the pENTER vector (Invitrogen). After virus packaged in HEK293T cells using Lipofectamine 3000, COL6A1, STAT1 or mock vectors were transfected into target cell lines. For knockdown of COL6A1 or STAT1, shRNA sequences targeting was cloned into a pLKO.1-TRC cloning vector. ShRNA-containing plasmids were packaged and transfected into target cell lines. Transfected cells used for overexpression or knockdown were selected with puromycin (5 µg/mL) for 2 weeks.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.