Culture cells and lung tissues were lysed with RIPA lysis buffer as previously described 27, 29, 30. Briefly, proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto polyvinylidene fluoride (PVDF) membranes. The membranes were incubated with primary antibody at 4 °C overnight. HRP-conjugated anti-mouse or anti-rabbit IgG was used as a secondary antibody for incubation for 1 h at room temperature. The reactive bands were detected by chemiluminescence (Advansta, CA, USA).

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