All statistical analyses were performed using GraphPad Prism 8 software (GraphPad Software). The Student t test, ×2 test, the Wilcoxon signed-rank test, was conducted as appropriate. Kaplan-Meier method was applied to measure the survival curves, and the log-rank test was used to assess the differences. Correlations were assessed by Pearson correlation analysis. P values less than 0.05 indicated statistical significance. *P < 0.05, **P < 0.01, ***P < 0.001.

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