0.3% formaldehyde was used to cross-link and glycine solution was used to quench the cells (Millipore). Sangon Biotech synthesized the biotin-labelled circDLC1 probes and control probes (Table S6). Biotin-labeled RNA pull-down was conducted according to the manufacturer's instructions of the EZ-Magna ChIRP RNA Interactome Kit (Millipore). Briefly, 1/5 associated RNA-protein complexes were separated for RNA purification, and the remaining were subjected to protein purification. RNA was purified with RNeasy Mini Kit (Qiagen Inc.). RT-qPCR was used to identify and quantify the enrichment of circDLC1. On the other hand, 4/5 associated RNA-protein complexes were treated with RNase A, RNase H and DNase to elute proteins. The purified proteins were subsequently analyzed by Western blot.

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