TGF-β enzyme-linked immunosorbent assay
This protocol is extracted from research article:
Reversible EMT and MET mediate amnion remodeling during pregnancy and labor
Sci Signal, Feb 11, 2020; DOI: 10.1126/scisignal.aay1486

Human amniotic fluid was collected at term vaginal deliveries (term labor), TNIL caesarian deliveries, pPROM, spontaneous preterm births, and a human/mouse TGF-β 1 ELISA Ready-SET-Go! ELISA (second generation) (Affymetrix eBioscience) was conducted following the manufacturer’s instructions. Standard curves were developed with recombinant protein samples of known quantities. Sample concentrations were determined by correlating the samples absorbance to the standard curve by linear regression analysis.

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