Cell shape index quantification
This protocol is extracted from research article:
Reversible EMT and MET mediate amnion remodeling during pregnancy and labor
Sci Signal, Feb 11, 2020; DOI: 10.1126/scisignal.aay1486

The cell shape index was determined for AEC and AMC cultures by evaluating one frame from each N (total of three images) per treatment for cell circularity using ImageJ software. The shape index was calculated using the following formula: SI = 4π × area/perimeter2, which is an established method that was originally reported to determine vascular cell shape (64). A circle would have a shape index of 1; a straight line, an index of 0.

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