To determine the expression levels of TAB1 and PGRMC2 after treatments, cells were collected and lysed using lysis buffer (QIAGEN). RNA was extracted using an RNeasy kit (QIAGEN) as per the manufacturer’s instructions. Total RNA (500 ng) was reverse-transcribed using a High-Capacity RNA-to-cDNA kit (Applied Biosystems). qRT-PCR was performed on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems) using Fast SYBR Green Master Mix (Applied Biosystems). The amplification thermal profile was 20 s at 95°C and 3 s at 95°C, followed by 30 s at 60°C (40 cycles). To confirm the presence of a single amplicon, a melt curve was carried out: 15 s at 95°C, 1 min at 60°C, 15 s at 95°C, and 15 s at 60°C. Changes in gene expression levels were calculated by using the ΔΔCt method. We used predesigned quantitative PCR assays from Integrated DNA Technologies (table S4).

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