Trichrome staining for collagen
This protocol is extracted from research article:
Reversible EMT and MET mediate amnion remodeling during pregnancy and labor
Sci Signal, Feb 11, 2020; DOI: 10.1126/scisignal.aay1486

Tissue sections were fixed in 4% paraformaldehyde for 48 hours and embedded in paraffin. Sections were cut at 5 μm thickness and adhered to a positively charged slide and attached by keeping them at 57°C for 45 min. Slides were deparaffinized using xylene; rehydrated with 100% alcohol, 95% alcohol, and normal saline (pH 7.4); and stained using the Masson’s trichrome method to identify collagen components. The amnion epithelium was identified by a single layer of cells, whereas the ECM was identified as the area in between the amnion epithelium and the chorion layer. Three microscopic fields were captured at ×10 and ×40 magnification.

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