Scratch assay and cell culture treatments
This protocol is extracted from research article:
Reversible EMT and MET mediate amnion remodeling during pregnancy and labor
Sci Signal, Feb 11, 2020; DOI: 10.1126/scisignal.aay1486

Passage 1 AECs were seeded at about 80% confluence in four-well coverslips and incubated at 37°C with 5% CO2 for 24 hours. AECs were then serum-starved for 1 hour, rinsed with sterile 1× PBS, and then scratched evenly down the middle of the well, in a straight line, with a 200-μl pipette tip (7). Cells were washed with sterile 1× PBS four times to remove any cell debris. Cell were then treated with TGF-β (15 ng/ml) or P4 (30ng/ml) for 1, 24, and 38 hours. Bright-field and confocal microscopy documented wound closure, morphology, and vimentin/CK-18 staining.

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