The appropriate promoter regions of the ARID5A, IRF1, MX2, and IL12B genes or the 3′UTR region of IL6 mRNA were amplified from human macrophage genomic DNA by PCR and subcloned into the pGL3 luciferase reporter vectors (Promega) using the In-Fusion HD kit according to the manufacturer’s protocol. Deletion constructs of the appropriate promoters were prepared with the KOD-Plus-Mutagenesis Kit according to the manufacturer’s instructions. U3A or HEK293T cells cultured in 24-well plates were cotransfected with the appropriate pGL3-luciferase plasmid or pGL3-luciferase control plasmid, together with the appropriate overexpression plasmid and the Renilla Luciferase control reporter (Promega). Transfections were performed with the Lipofectamine 3000 kit (Thermo Fisher Scientific, L3000015) or Lipofectamine LTX (Thermo Fisher Scientific, 15338100) for U3A and HEK293T cells, respectively. Forty-eight hours later, the luciferase activity in the cell lysates was measured with the Dual Luciferase Reporter Assay System, and the data were normalized to Renilla luciferase activity. Primers used for PCR-based amplification are listed in table S1.

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