To determine the effects of inhibiting mitochondrial function on SOCE in LS8 cells, we maximally depleted the ER stores by preincubating cells for 20 min with thapsigargin (1.25 μM). Cells were also loaded with Fura 2-AM (1 μM). To disrupt mitochondrial complexes of the ETC, we treated cells for 10 min with rotenone (2 μM) and oligomycin (1 μM) to block complexes I and V, respectively, before the re-addition of 2 mM Ca2+ to induce SOCE.

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