Small-molecule screening was performed using the ICCB-Longwood Screening Facility ( GFP-expressing cells were seeded into black-sided 384-well plates at 600 cells per well using a Multidrop Combi Reagent Dispenser (Thermo Fisher Scientific, no. 5840300) in a final volume of 30 μl per well. After 24 hours, a Seiko Compound Transfer Robot was used to pin transfer 100 nl of each library plate well into the cell-containing plates. Forty-eight hours later, the GFP signal was measured as a proxy for cell number using an Acumen Laser Scanning Cytometer. The Ludwig Anti-Cancer Library of compounds was used and contains ~400 compounds in 10-point concentration curves ranging from 5 nM to 33.3 μM. The average Z′ value for this screening setup was 0.75 when using actinomycin D as the positive control.

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