Phosphoproteomics of rat glomeruli was essentially performed as previously described (38). Glomerular peptides (100 μg) were subjected to desalting using Oasis HLB columns (Waters). Desalted peptides were dried down and resuspended in 20% acetic acid. Phosphopeptides were enriched using Fe-NTA phosphopeptide enrichment resin columns (Thermo Fisher Scientific) as previously described (38), and StageTips cleanup was performed.

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