Glomeruli were solubilized in 8 M urea containing 10 mM tris and protease inhibitor (Roche cOmplete) as well as phosphatase inhibitor cocktail 1× (Thermo Fisher Scientific). Protein abundance was determined with the BCA (Thermo Fisher Scientific), and 50 μg of protein was further processed for proteomic analysis. In brief, proteins were reduced using 5 mM dithiothreitol and 10 mM iodoacetamide. Trypsin (Promega, MS grade) was added at a 1:200 (w/w) ratio, and proteins were digested overnight. The next day, digested peptides were acidified and cleaned up using stop and go in-tip cleanup (StageTips).

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