Targeted metabolomic analysis was performed on a triple-quadrupole (QQQ) mass spectrometer (Agilent Triple Quadrupole 6490, San Diego, CA) coupled to a high-performance liquid chromatography (HPLC) system (1290 Infinity, Agilent Technologies) coupled to ion funnel. A ZIC-pHILIC (Sequant column; 2.1 × 150 mm) was used for separation. Cycle time was 500 ms. Collision energies and product ions (MS2 or quantifier and qualifier ion transitions) were optimized. Electrospray ionization source conditions were set as follows: gas temperature, 250°C; gas flow, 12 liters/min; Nebulizer, 20 psi; sheath gas temperature, 350°C; cap voltage, 2000 V; and nozzle voltage, 1000 V. The gradient consisted of buffer A and buffer B. Buffer A was 95:5 H2O:acetonitrile, 20 mM NH4OAc, 20 mM NH4OH (pH 9.4). Buffer B was acetonitrile. The gradient with A/B ratios were as follows: T0, 10:90; T1.5, 10:90; T20, 60:40; T25, off. Five microliters was injected. The used transitions for metabolites can be found in table S2. Metabolite identity was also verified by the addition of deuterated isotope–labeled standards. To quantify oxolipids, the manufacturer’s suggested transitions were followed (Cayman Chemical, item no. 192228).

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