The following antibodies were raised in sheep and affinity-purified on the appropriate antigen by the Division of Signal Transduction Therapy Unit at the University of Dundee: KCC2A phospho-Thr906 [SAYTYER(T)LMMEQRSRR (residues 975 to 989 of human KCC3A) corresponding to SAYTYEK(T)LVMEQRSQI (residues 899 to 915 of human KCC2A)] (catalog no. S959C), KCC2A phospho-Thr1007 [CYQEKVHM(T)WTKDKYM (residues 1032 to 1046 of human KCC3A) corresponding to TDPEKVHL(T)WTKDKSVA (residues 998 to 1014 of human KCC2A)] (catalog no. S961C), and KCC2 phospho-Ser940 (catalog no. NBP2-29513). Pan-KCC2 total antibody (residues 932 to 1043 of human KCC2) was purchased from NeuroMab (catalog no. 73-013). Anti–β-tubulin III (neuronal) antibody was purchased from Sigma-Aldrich (catalog no. T8578). Secondary antibodies coupled to horseradish peroxidase used for immunoblotting were obtained from Pierce. Immunoglobulin G used in control immunoprecipitation experiments was affinity-purified from preimmune serum using protein G–Sepharose.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.