All cell adhesion experiments were performed using previously described protocols with modifications (30, 57). Briefly, coverslips were charged using piranha solution for 1 hour at 25°C, washed three times with water, and dried at 100°C for about 10 min. Then, coverslips were treated with 20% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) in isopropanol for 90 min, washed three times with isopropanol, and dried at 100°C for about 30 min. For FN coating, silanized coverslips were incubated with bovine plasma FN (10 μg/ml; Invitrogen) in 1× PBS for 60 min at 37°C. For N-cadherin and E-cadherin substrate generation, silanized coverslips were initially incubated with goat anti-human IgG (10 μg/ml; Sino Biological) in 1× PBS for 1 hour at 37°C. Coverslips were subsequently incubated in human N-cadherin Fc (Sino Biological) or E-cadherin Fc (Sino Biological) in 1× PBS at a concentration of 10 μg/ml for 60 min at 37°C.

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