Male C57BL/6J mice and 3x Tg-AD mice (8 to 12 weeks old) were given daily by intraperitoneal injections with STZ (60 mg/kg body weight in 0.1 M citrate buffer) (Sigma-Aldrich) for 5 days to induce diabetes. Control mice were injected with buffer alone. Blood glucose levels were examined 7 days after the final STZ injection by measuring glucose concentration with a glucometer (Accu-Chek instant, Boehringer Mannheim Corporation). Mice with blood glucose levels greater than 300 mg/dl were considered diabetic. At the time of harvest, 19 days after the first STZ injection, blood glucose measurements were again obtained, mice were humanely euthanized, and brains were harvested for analyses. All experiments involving animals were performed according to the protocols approved by the Seoul National University Institutional Animal Care and Use Committee guidelines.

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