Immunohistochemical studies of brain sections from patients with AD were described previously (64). The hippocampi of patients with AD were fixed in paraformaldehyde (PFA) for 48 hours before serial coronal sections and cut on a freezing microtome. The sections were placed on the slides and refixed in 4% PFA. Samples were incubated in 0.1% Triton X-100 for 20 min and then in 1% bovine serum albumin for 1 hour before the application of a primary antibody. Blocked sections were incubated with anti-SERP1 (1:200), anti-APH1A (1:300), anti-GFAP (1:200), or anti-SYP (1:200) antibodies; washed; and incubated with fluorescein isothiocyanate–conjugated and tetramethyl rhodamine isothiocyanate–conjugated secondary antibodies (the Jackson laboratory). Samples were washed, mounted, and observed under a Zeiss LSM700 microscope.

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