Overlay assay was performed as described previously (63). SERP1 protein (10 μg), which was expressed in E. coli and purified, was resolved by SDS-PAGE and transferred to nitrocellulose membranes. After blocking, the membranes were incubated at 4°C for 12 hours with whole-brain lysates of 3-month-old C57BL/6 mice. Membranes were then processed to Western blotting as mentioned above.

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