General immunoprecipitation was performed using 1% Triton X-100 buffer (50 mM tris, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate, and protease inhibitor cocktail). Immunoprecipitation of γ-secretase protein complex was performed using 1% CHAPS buffer [25 mM Hepes (pH 7.4), 150 mM NaCl, 2 mM EDTA, 1% CHAPS, and protease inhibitor cocktail] or 1% digitonin buffer [25 mM Hepes (pH 7.4), 150 mM NaCl, 2 mM EDTA, 1% digitonin, and protease inhibitor cocktail]. Cell lysates were prepared by sonication in Triton X-100, digitonin, or CHAPS buffer. After brief centrifugation, the supernatants were incubated with anti-HA, anti-GFP, anti-SERP1, anti-PS1, or anti-APH1A antibody at 4°C overnight and then pulled down using Protein G Sepharose beads (GE Healthcare).

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