Luciferase reporter γ-secretase activity assay was as described (25). HEK293T cells were cotransfected with pC99-GVP, pUAS-luciferase, pβ-galactosidase, and experimental control genes with or without 1 μM Compound E. After 24 hours, cell extracts were analyzed by luciferase assay following the manufacturer’s instructions (Promega). The luciferase activity was normalized by β-galactosidase activity for transfection efficiency.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.