Male mice (8 to 10 weeks old) were administered ConA at a dose of 15 mg/kg in sterile PBS by tail vein injection. After 12 hours, the mice were anesthetized with isoflurane, bled by cardiac puncture, and euthanized by rapid cervical dislocation. The blood was collected in tubes containing 50 μl of 5 mM EDTA. To obtain plasma, the blood was centrifuged at 3000g for 5 min at 4°C. RBCs were lysed with hypotonic ACK lysis buffer (150 mM NH4Cl, 10 mM KHCO3, and 100 nM Na2EDTA). The remaining leukocytes were lysed in 1 ml of TRIzol for gene expression analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.