After stimulation, cells were lysed in radioimmunoprecipitation assay buffer [50 mM tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 mM sodium fluoride, and 25 mM sodium pyrophosphate] supplemented with phosphatase inhibitors (Roche). The protein lysates were mixed with NuPAGE LDS sample buffer (Novex), resolved by SDS–polyacrylamide gel electrophoresis, and analyzed by Western immunoblotting with anti–phospho-TBK1 antibody (D52C2Cell Signaling Technology) and anti-TBK1 antibody (D1B4, Cell Signaling Technology).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.