MDMs, THP1 cells, or murine BMMs (1 × 105 cells) were stimulated with NETs (100 μg/ml) for 15 hours. For THP1 cells and murine BMMs, the transfection reagent DOTAP (150 μg/ml, Carl Roth) was added. The inhibitors NEi (20 μM, AdipoGen), sivelestat (1 μM, Sigma-Aldrich), K777 (10 μM, AdipoGen), and Z-VAD-FMK (20 μM, InvivoGen) were added to the cells before they were activated with NETs. Cells were transfected with pdAdT (1 μg/ml) as a positive control with DOTAP.

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