Chlorogenic acid (catalog no. C3878), H2O2, 6-OHDA, and PARP inhibitor 3AB were purchased from Sigma-Aldrich (Merck KGaA). Tamoxifen (Selleck Chemicals) and Akt1 inhibitor MK2206 (catalog no. 11593, Cayman Chemical) were also used. The following primary antibodies were used: mouse antibody to RNF146 (catalog no. N201/35, 1:5000, NeuroMab), rabbit antibody to GFP (catalog no. 2956, 1:5000, Cell Signaling Technology), mouse antibody to PAR [for Western blot (WB), catalog no. 4335-MC-100, 1:3000, Trevigen; for immunofluorescence (IF), catalog no. sc-56198, 1:3000, Santa Cruz Biotechnology], rabbit antibody to tyrosine hydroxylase (catalog no. NB300-109, 1:2000, Novus Biologicals), rabbit antibody to CREB (catalog no. 9197S, 1:3000, Cell Signaling Technology), rabbit antibody to pCREB (catalog no. 9198S, 1:3000, Cell Signaling Technology), mouse antibody to Akt1 (catalog no. 2967S, Cell Signaling Technology), rabbit antibody to pSer473-Akt1 (catalog no. 9018S, Cell Signaling Technology), rabbit antibody to Akt2 (catalog no. 5239, Cell Signaling Technology), rabbit antibody to pSer474-Akt2 (catalog no. 8599, Cell Signaling Technology), rabbit antibody to AIF (catalog no. ab32516, Abcam), and rabbit antibody to tubulin β-3 (Tuj-1, catalog no. 802001, 1:1000, BioLegend). The following secondary antibodies were used: horseradish peroxidase (HRP)–conjugated sheep antibody to mouse immunoglobulin G (IgG) (catalog no. RPN4301, 1:5000, GE Healthcare), HRP-conjugated donkey antibody to rabbit IgG (catalog no. RPN4101, 1:5000, GE Healthcare), biotin-conjugated goat antibody to rabbit IgG (catalog no. BA-1000, 1:1000, Vector Laboratories), HRP-conjugated mouse antibody to β-actin (catalog no. A3854, 1:10000, Sigma-Aldrich), Alexa Fluor 568–conjugated donkey antibody to rabbit IgG (catalog no. A10042, Invitrogen), Alexa Fluor 488–conjugated goat antibody to rabbit IgG (catalog no. A11008, Invitrogen), Alexa Fluor 568–conjugated donkey antibody to mouse IgG (catalog no. A10037, Invitrogen), Alexa Fluor 488–conjugated donkey antibody to mouse IgG (catalog no. A21202, Invitrogen), and Alexa Fluor 405–conjugated goat antibody to rabbit IgG (catalog no. ab175652, Abcam).

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