Human EA.hy926 Cas9+ cells (6) were maintained in DMEM supplemented with 10% (v/v) low-endotoxin FBS. Cells were plated overnight at ~1.5 × 105/ml in 100 μl in 96-well plates and stimulated the following day with 500 μM etoposide or transfected with LPS (5 μg/ml) and 0.2% (v/v) Lipofectamine LTX (Thermo Fisher Scientific) in Opti-MEM I medium.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.