Human EA.hy926 Cas9+ cells (6) were maintained in DMEM supplemented with 10% (v/v) low-endotoxin FBS. Cells were plated overnight at ~1.5 × 105/ml in 100 μl in 96-well plates and stimulated the following day with 500 μM etoposide or transfected with LPS (5 μg/ml) and 0.2% (v/v) Lipofectamine LTX (Thermo Fisher Scientific) in Opti-MEM I medium.

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