The following reagents were used to treat cells: Ultrapure LPS (Escherichia coli O111:B4, InvivoGen), Pam3CSK4 (InvivoGen), IFN-α (PBL Assay Science), IFN-β (PBL Assay Science), IFN-γ (eBioscience), ATP (Sigma), Cyto-c (bovine heart Cyto-c, Sigma), etoposide (Cell Signaling Technology), and FasL (MegaFasL, AdipoGen). Antibodies used in this study were specific for the following targets: caspase-1 (clone 4B4, Genentech), caspase-4 (clone 4B9, Enzo), caspase-11 (clone 17D9, Novus Biologicals), GSDMD [clone 17G2G9 (9), Genentech], IRF1 (clone D5E4, Cell Signaling Technology), and IRF2 (raised against full-length recombinant mouse IRF2, mouse IgG2b, clone 25, Genentech). In addition, horseradish peroxidase (HRP)–conjugated antibody against β-actin (AC-15, Novus Biologicals) and the FLAG epitope (M2 HRP, Sigma) were used.

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