Graphs were made and statistical analyses were performed using GraphPad Prism (GraphPad Software Inc., La Jolla, CA). Data were expressed as means ± SEM. Comparison of survival curves used a one-way analysis of variance (ANOVA)–like nonparametric Kruskal-Wallis test and a multiple comparison test where adjusted P value of Wilcoxon rank sum test was used to evaluate significance. Tumor growth curves were fit using a second-order polynomial nonlinear regression. For analysis of three or more groups, ANOVA tests were performed with a Tukey’s or Sidak’s multiple comparisons test. Analysis of differences between two normally distributed paired test groups was performed using a Student’s t test. Analysis of differences between two normally distributed unpaired groups was performed using an unpaired two-tailed t test. Statistical outliers were identified using the ROUT method (Q = 1 to 10%). P values were considered statistically significant if P < 0.05.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.