Necropsy of subcutaneous animals occurred after euthanasia. Tumors were excised; formalin was fixed for 48 hours and stored in 70% ethanol until histological staining. Necropsy of window chamber animals occurred after 14 days at which point the titanium window chamber was removed; the skin was excised and frozen in optimal cutting temperature (OCT) media. Before histological analysis, tumor samples were formalin-fixed for 48 hours and stored in 70% ethanol until histological staining was performed. All tumor samples were sent to the Research Histology, Pathology, and Imaging Core at UT MD Anderson Cancer Center where they were sectioned, stained, and analyzed.

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