The κB5→IκBα-FLuc and κB5→FLuc plasmids were previously described (49, 50). The FUW-FLG plasmid encodes a fusion of click beetle green (CBG) and EGFP (enhanced green fluorescent protein) proteins driven by the human ubiquitin C promoter (CBG) within an established lentiviral backbone, as previously described (73). The pFLuc control plasmid was generated as previously described (74). The GFP-IRES-GFP lentivirus was a gift from R. DePinho (UT MD Anderson Cancer Center, TX, USA). The pDendra2-N and pLVX-IRES-Puro were purchased from Clontech (Clontech, Mountain View, CA, USA). A Cignal lenti NFκB→FLuc reporter was purchased from Qiagen (SABiosciences, Frederick, MD, USA).

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