Mouse and human IL-1 to IL-10 and IL-12 were quantified using the appropriate mini enzyme-linked immunosorbent assay (ELISA) development kit (PeproTech) according to the manufacturer’s instructions. Plates were read at 405 nm with wavelength correction set at 650 nm using a Varioskan LUX multimode microplate reader (Thermo Fisher Scientific).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.