WT and β-arrestin1/2 KO CHO cells were seeded in white opaque 96-well plates and transfected with SRF-RE, GHSR1a, and either empty vector or MRAP2. The day after transfection, media was replaced with 60 μl of serum-free medium containing the indicated concentration of ghrelin. Cells were incubated at 37°C for 4 hours before adding 60 μl of substrate solution containing 200 mM tris-HCl, 10 mM MgCl2, 300 μM adenosine triphosphate, 1% Igepal, 12.2 mM coenzyme A, and d-luciferin (150 μg/ml). Luminescence was measured on a SpectraMax i3 plate reader (Molecular Devices).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.